Extended Data Fig. 7: Effects of chronic 4-AP incubation on zebrafish. | Nature Neuroscience

Extended Data Fig. 7: Effects of chronic 4-AP incubation on zebrafish.

From: Functionally distinct subgroups of oligodendrocyte precursor cells integrate neural activity and execute myelin formation

Extended Data Fig. 7: Effects of chronic 4-AP incubation on zebrafish.

a, Minimum intensity projections of a 2 min time-lapse of fish freely swimming in a 3 cm petri dish in different treatment conditions (n = 6, n = 7, n = 3 and n = 3 animals in control, 4-AP, TTX and 4-AP+TTX conditions, three independent experiments). b, Traces of GCaMP transients from Tg(elavl3:h2b-GCaMP6s) zebrafish at 4 d.p.f. and after overnight incubation in 0.1 mM 4-AP and before and after 10 µM TTX (seven animals per condition in two experiments). c, Confocal images of Tg(mfap4:memCerulean),Tg(olig1:nls-mApple) zebrafish at 4 d.p.f. after treatment with 0.1 mM 4-AP, 0.5 mM 4-AP, or Danieau’s solution as a control. Transmitted light images show spinal cord morphology and tissue integrity after drug treatment. Scale bars, 100 µm. The graph shows the number of macrophages that accumulate in a 400 µM length of spinal cord of Tg(mfap4:memCerulean) zebrafish after 1 d of control (2 ± 0.25/2 cells), 0.1 mM (2 ± 1/2 cells) and 0.5 mM (3 ± 0.25/2 cells) 4-AP treatment (median ± 25%/75% percentiles). P = 0.43 (control versus 0.1 mM 4-AP), P = 0.03 (control versus 0.5 mM 4-AP), Kruskal–Wallis test, test statistic=3.003, n = 16, n = 19 and n = 8 animals in three experiments. d, Representative images of Tg(mbp:nls-EGFP),Tg(olig1:nls-mApple) zebrafish in control treatment and after 2 d of 0.1 mM 4-AP treatment (see Fig. 7e for n values). Scale bar, 20 µm.

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