Extended Data Fig. 8: SC projects back to Pf and VM in a topographical fashion.
From: Anatomically segregated basal ganglia pathways allow parallel behavioral modulation
a, Overlap of SNr axons labelled via anterograde tracing in striatum (AAV1.Cre, see Fig. 2e) and cortical axons. Scale bar, 500um. bottom, from Allen Institute for Brain Science. Allen Mouse Brain Connectivity Atlas (2011). Available from https://connectivity.brain-map.org/. b, Experimental protocol for labelling medial and lateral SC. Two anterograde tracers (AAV1.Cre and AAV1.Flpo) were injected in ACA/tjM1 respectively, followed by AAV.DIO.TdTom or AAV.fDIO.EYFP in medial or lateral SC. This allowed labelling of mSC and lSC without leaking into other regions outside SC. c, Example coronal section showing the injection site in SC (mSC in red, lSC in green). Similar results were obtained in n = 3 mice. Scale bar, 1mm. d, Example coronal sections showing the axonal targets of mSC and lSC. We observed topography in both Pf and VM, similar to SNr topography (see main Text). Similar results were obtained in n = 3 mice. Scale bar, 1mm.
