Extended Data Fig. 7: In vitro targeting of astrocytes and morphological analyses. | Nature Neuroscience

Extended Data Fig. 7: In vitro targeting of astrocytes and morphological analyses.

From: Tau accumulation in astrocytes of the dentate gyrus induces neuronal dysfunction and memory deficits in Alzheimer’s disease

Extended Data Fig. 7

a, Confocal micrographs of cultures infected with LV-G1-GFP showing the co-localization of GFP and GFAP (red, left panel), NeuN (red, middle panel) or Iba1 (red, right panel). b, Histogram showing the proportion of infected cells that co-expressed GFP with GFAP, Iba1 or NeuN, c, Confocal micrographs of cultures co-infected with LV-G1-GFP or LV-G1-1N3R+LV-G1-GFP or LV-G1-1N4R+LV-G1-GFP. d, Histogram showing the proportion of cells that were co-infected in the LV-G1-1N3R+LV-G1-GFP or LV-G1-1N4R+LV-G1-GFP conditions. e, Confocal micrographs of astrocytes after infection with LV-G1-GFP or LV-G1-1N3R+LV-G1-GFP or LV-G1-1N4R+LV-G1-GFP. Images are overlaid with a scaffold of the cell’s morphology. f-k, Violin graphs of the astrocytes’ (f) soma area, (g) total territory area, (h) total length of processes, (i) number of branching points, j, number of segments, (k) number of terminal points. N=cultures/cell per culture. (b): LV-G1-CFP: 4/203. (d): LV-G1-1N3R: 4/102 and LV-G1-1N3R: 4/97. (f): LV-G1-CFP: 4/70, LV-G1-1N3R: 4/81 and LV-G1-1N3R: 4/55. (g-k): LV-G1-CFP: 4/12, LV-G1-1N3R: 4/12 and LV-G1-1N3R: 4/12. Data are presented as the mean ± SEM. One-sided ANOVA with Tukey’s post-hoc test (b, f-k) and Mann-Whitney two-tailed t-test (d). Data are presented as the mean ± SEM. Scale bars: 50 µm (c), 20 µm (a, e).

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