Extended Data Fig. 4: Identification of GABAARs using sub-tomograms mixed with sub-tomograms on presynaptic membrane.
From: Mesophasic organization of GABAA receptors in hippocampal inhibitory synapses
a, Steps for identifying GABAARs from dataset mixed with sub-tomograms on presynaptic membrane. a1, Electron tomographic slice of an inhibitory synapse. a2, Electron tomographic slices superposed with sampling points on postsynaptic membrane (top) or presynaptic membrane (bottom). b, Sub-tomogram sampling points on postsynaptic membrane (top) or presynaptic (bottom) after each step. Red points are sampling points that will be discarded in the next step. For a and b, n = 72 postsynaptic membranes, and n = 4 presynaptic membranes. c, 3D classification and refinement of sub-tomograms on 72 postsynaptic membranes and 4 presynaptic membranes. d, Receptor concentration from Analysis II (analysis of sub-tomograms mixed with presynaptic sub-tomograms) versus receptor concentration from Analysis I (analysis of sub-tomograms without presynaptic sub-tomograms). e, Concentration of identified receptors on postsynaptic membranes in Analysis I, Analysis II, and falsely identified receptors on presynaptic membranes in Analysis II for the 4 selected synapses.
