Extended Data Fig. 3: RNA sequencing reveals differentially expressed gene clusters in neuroblasts from young and aged dentate gyrus.

Single-cell suspensions were prepared from dissected dentate gyrus of male young (3 months) and aged (18 months) DCX-mRFP reporter mice. Thereafter, mRFP⁺ neuroblasts were sorted via flow cytometry. a, Heat map shows RNA transcriptomes of 1,101 differentially expressed genes from neuroblasts of young and aged dentate gyrus. The relative abundance of transcripts is indicated by the color (red, high; blue, low). b, Enrichment analysis was performed to measure the significance of altered gene expression by Gene Ontology annotations. The enrichment of pathways was analyzed by Fisher exact test. c, Real-time PCR analysis of selected genes that reflect neuroblast functions, such as cell growth and death, cell cycle, immune regulation, etc, in aged neuroblasts versus young neuroblasts. n = 3 per group. d, Bubble diagram displays enriched pathways based on function in the KEGG database. Pathway categories with FDR <0.05 were reported. e. KEGG database was used to build the network of differentially expressed genes of aged/young neuroblasts according to the relationship among mRNA, miRNA and proteins in the database. Error bars represent s.e.m.