Extended Data Fig. 4: Firing rate of excitatory and inhibitory units during T-maze in Control and GFAP/PFCΔGb mice.
From: GABAergic signaling to astrocytes in the prefrontal cortex sustains goal-directed behaviors
(a) Representative waveforms for putative excitatory units (EU, blue) and inhibitory units (IU, orange) and corresponding auto-correlograms. (b), (c) BW plots showing information content analysis for EU (b) and IU (c). Note the reduced magnitude of information content index for GFAP/PFCΔGb mice. (d), (e) Left, histograms of normalized CV probability distribution (control black line, GFAP/PFCΔGb purple area). Right, percentage of EU (d) and IU (e) displaying and enhanced activity (open boxes), decreased activity (filled boxes) or no changes in firing rate (dashed boxes) based on CV values during correct trials (>1.5 deviation from median population). Note the reduced magnitude of open boxed in GFAP/PFCΔGb mice for excitatory units indicating a minor population of cells increasing their firing rate after entering the DZ (values for Z-test values indicated in panel d, e). (f), (g) Left, Histograms showing the diversity of firing rates across the neuronal populations, as reflected in a lognormal distribution. GFAP/PFCΔGb mice (purple histogram) showed right-shifted distributions towards higher values of firing rate for both EU (f) and IU (g). Insets, BW plots for the mean firing rate of all units along the trials (EU, n = 490 for Control; n = 396 for GFAP/PFCΔGb; 4 mice for each group; P < 0.001; One Way ANOVA two-sided; IU, n = 110 for Control; n = 109 for GFAP/PFCΔGb; P = 0.021; KW test). Right, Mean Z-score of the firing rate for EU and IU populations, respectively, including correct and incorrect trials for Control and GFAP/PFCΔGb mice. Data are shown as mean ± SEM (shaded areas). Note the disruption of firing pattern during T-maze performance in GFAP/PFCΔGb mice for both EU (P = 0.01; One Way ANOVA two-sided) and IU (P = 0.016; One Way ANOVA two-sided). * P <0.05; *** P <0.001 (see Source Data file). Data shown are mean±SEM. Box plots indicate median and whiskers indicate the 25th and 75th percentiles, respectively.
