Extended Data Fig. 6: PV-driven IPSCs potentiation onto PCs relies on presynaptic mechanisms.
From: GABAergic signaling to astrocytes in the prefrontal cortex sustains goal-directed behaviors
(a) Relative changes of IPSC amplitude over time (red arrow denotes PV stimulation), showing different outcomes after PV depolarization. Right, BW plot showing changes in IPSC amplitude after <2 min PV stimulation for both groups (P = 0.030; One-way ANOVA two-sided). Inset, Pie plot representing the percentage of PCs showing changes (79%, gray) vs no changes (21%, blue) in IPSCs amplitude. Note the preponderance of PC responses showing IPSCs potentiation. (b) Relative changes of the coefficient of variation (CV) after <2 min PV depolarization for PCs showing IPSCs potentiation (n = 16), suggesting a presynaptic locus for IPSC modulation. (c) Representative traces of a connected PV-PC pair (current clamp recordings for PV interneuron, voltage clamp recordings for PCs) showing unitary synaptic responses onto PCs. Note the increase of unitary IPSCs responses after the PV stimulation protocol. Right, Relative changes of synaptic parameters elicited by the PV stimulation protocol: success rate (P = 0.022), synaptic efficacy (P = 0.008) and synaptic potentiation (P = 0.360; KW test). * P <0.05; ** P <0.01 (see Source Data file). Data shown are mean±SEM. Box plots indicate median and whiskers indicate the 25th and 75th percentiles, respectively.
