Extended Data Fig. 10: mGluR1 activation contributes to cognitive performance.
From: GABAergic signaling to astrocytes in the prefrontal cortex sustains goal-directed behaviors
(a) Left, scheme of local injection of LY367385 (100 μM) into mPFC to selectively block mGluR1 receptors. Right, stereoscopic image showing bilateral cannula positions for drug delivery into the PFC. Scale bar, 500 μm. (b) Top, representative traces of behavioral responses in T-maze at day 4 for wildtype mice treated with saline and LY367385 (100 μM) to selectively block mGluR1 receptors. Bottom, temporal profile of learning task. Data are shown as mean ± SEM (shaded areas). (c) BW plot showing the average performance for control (n = 15), saline-treated mice (n = 4), and LY367385-treated mice (n = 4) during training. (d) Contingency table for data statistics shown in c. Heat map denote significant levels; Two Way ANOVA two-sided (see Source Data file). (e) Confocal images of GAD67 labeling (blue), mDlx-GFP (green), s100 (gray) and Cre-mCherry (red), showing the selective expression of mDlx-GFP in GABAergic cells and Cre-mCherry in astrocytes in GFAP/PFCΔGb mice. Scale bar, 25 μm. Data shown are mean±SEM. Box plots indicate median and whiskers indicate the 25th and 75th percentiles, respectively. (f) Scheme summarizing data from this study highlighting the role of astrocytes in goal-directed actions.
