Extended Data Fig. 2: Astrocytic downregulation of GABABR1s altered behavioral performance.
From: GABAergic signaling to astrocytes in the prefrontal cortex sustains goal-directed behaviors
(a) Histological verification of bilateral cre expression (red) in astrocytes in mPFC. Scale bar, 200 μm. (b) Correlation between cre-transfected area and T-maze performance. Dots represented the number of incorrect trials at day 4 for individual GFAP/PFCΔGb mice (linear fitting curve, black line). (c) BW plots for behavioral sessions in T-maze after training (recall sessions, post day 6) for Control (black) and GFAP/PFCΔGb (purple) (n = 10 recall sessions; n = 4 mice for each group; P <0.001; One Way ANOVA two-sided). (d, e) Anxiety levels measured for control and GFAP/PFCΔGb mice. Note the lower anxiety levels showed by mice with downregulated GABABRs specifically in astrocytes. (d) Representative traces for Control and GFAP/PFCΔGb mice in an open field test. BW plot showing the increased exploration index for GFAP/PFCΔGb mice (n = 4 mice for each group; P = 0.044; One Way ANOVA two-sided), while average of running speed during exploration was similar in both mice. (e) Scheme and activity traces in an elevated plus maze (EPM) for Control and GFAP/PFCΔGb mice. Note that GFAP/PFCΔGb mice had more entries into the open arms than Control. Right, EPM exploration index (n = 4 for each condition; P = 0.037; One Way ANOVA two-sided). (f) Scheme and activity traces of object in place (OIP) test for Control and GFAP/PFCΔGb mice. BW plot for exploration index showing a reduced working memory performance in GFAP/PFCΔGb mice (n = 5 for each condition; P = 0.019; One Way ANOVA two-sided). Right, BW plot for the exploration time in the arena for both mice. * P <0.05; *** P <0.001 (see Source Data file). Box plots indicate median and whiskers indicate the 25th and 75th percentiles, respectively.
