Extended Data Fig. 5: Immune landscape in the cerebral cortex following cerebrovascular injury in Cx3cr1gfp/wtCcr2rfp/wt mice.
From: Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury
Quantification of these flow cytometric experiments is provided in Fig. 3c,f, gating strategy in Supplementary Fig. 1B. The panel used for these experiments includes: Cx3CR1-GFP, Ly6C BB790, MHCII BV480, CD11b BV570, CD115 BV605, CD24 BV650, CD11c BV785, P2RY12 PE, CCR2-RFP, Ter-119 PE/Cy5, CD206 PE/Cy7, CD45 BUV395, CD4 BUV496, Ly6G BUV563, CD19 BUV661, CD44 BUV737, CD8 BUV805, F4/80 APC-R700, TCRb APC/Cy7 and live/dead fixable blue cell staining kit. Plots were pre-gated for CD45 + Ter119- live cells and subsequently analyzed using an unsupervised clustering algorithm to group data into subpopulations (PhenoGraph) and visualized using UMAP. For each experiment, the first row depicts the concatenated samples of 4 independent mice per group, and the legend shows the combined phenograph clusters corresponding to different immune cell populations. The second and third rows show six representative heatmaps of different markers used to identify the different immune cell populations. a, Immune landscape at 1 d and 6 d post-injury compared to uninjured mice. b, Immune landscape 1 d after injury in mice treated with bolus αLFA1/VLA4 or isotype control antibodies relative to uninjured mice.
