Extended Data Fig. 2: Cortical spine loss in c-MS mice compared to mice immunized only with MOG or injected only with cytokines.
a, Spine density along the apical dendrite of layer V pyramidal neurons in healthy control (Ctr) mice and MOG immunized animals without cytokine injection (n = 21 neurons from 7 and 8 mice in each group respectively; RM two-way ANOVA, P = 0.4907). b, Spine density along the apical dendrite of layer V pyramidal neurons in healthy control mice (n = 21 neurons from 7 mice) and non-immunized mice 3d after cytokine injection analysed ipsi- and contralateral to the injection site (n = 8 and 13 neurons from 10 mice respectively; RM two-way ANOVA, P = 0.1457). c, Spine density along the apical dendrite of layer V pyramidal neurons in healthy control (n = 21 neurons from 7 mice) and c-MS d3 animals analysed ipsi- and contralateral to the injection site (n = 17 and 11 neurons 8 mice respectively; RM two-way ANOVA, P < 0.0001, followed by Bonferroni’s multiple comparisons test, Ctr vs. c-MS ipsi: P < 0.0001, Ctr vs. c-MS contra P < 0.0001; data derived from Fig. 1g). The same Ctr group as in Fig. 1g replotted in all three graphs. Data represented as mean and the error bars indicate SEM. See Source Data file for individual data points and further statistical parameters. *** P < 0.001.
