Extended Data Fig. 5: Mo-TAM heterogeneity in GBM tumors. | Nature Neuroscience

Extended Data Fig. 5: Mo-TAM heterogeneity in GBM tumors.

From: Single-cell profiling of myeloid cells in glioblastoma across species and disease stage reveals macrophage competition and specialization

Extended Data Fig. 5: Mo-TAM heterogeneity in GBM tumors.The alternative text for this image may have been generated using AI.

a, Overview of Mo-TAM subsets in human ND GBM tumors, with 5 subclusters that were discerned: (1) Transitory Mo-TAM (green), (2) Phagocytic/lipid signature Mo-TAM (purple), (3) Hypoxic Mo-TAM (blue), (5) SEPP1+ Mo-TAM (pale orange), and (6) IFN signature Mo-TAM (pink). b, UMAP plots showing the expression of key signature genes that are differential between the various subsets. Dashed lines encircle the subsets as annotated in panel a. c, d, DE analysis in human ND Mo-TAMs. c, Volcano plot showing genes that are DE between Hypoxic Mo-TAMs (cluster 3) and SEPP1+ Mo-TAMs (cluster 5). d, Volcano plot showing genes that are DE between Phagocytic/lipid Mo-TAMs (cluster 2) and SEPP1+ Mo-TAMs (cluster 5). In red are shown genes with -log10(adjusted P) > 20, log2(FC) > 0.5; P value adjustment was performed using Bonferroni correction. e, f, Pie charts showing the percentage of Mo-TAM subsets in human ND tumors (e), and human recurrent tumors (f), per individual patient sample. g, Bar graphs showing the mean normalized expression of selected genes in ND SEPP1+ Mo-TAMs (ND cluster 5), Recurrent SEPP1low Mo-TAMs (R cluster 4) and Recurrent SEPP1high Mo-TAMs (R cluster 5), for the individual patient samples. Bar graphs represent mean ± s.e.m. Significance was evaluated using an ordinary One-way ANOVA and Tukey’s multiple comparisons tests. ns P > 0.05; * P < 0.05; ** P < 0.01. Exact P values can be found in Supplementary Table 19. h, Pie charts showing the percentage of Mo-TAM subsets in mouse GL261 tumors per individual mouse sample.

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