Extended Data Fig. 5: Mgl1+ epineurial macrophages express Lyve1 and are often located in close contact with blood vessels.

a, Expression of Lyve1 on snMacs subsets measured by flow cytometry. b, Representative histograms showing the expression of Lyve1 on Mgl1^- endoneurial snMacs (orange) and Mgl1⁺ epineurial snMacs (blue) over FMO controls (grey). (a,b), Data are representative of five individual animals. Cells from all individual mice were pooled after recording in one plot. c, Confocal microscopy was used to verify the location of Lyve⁺ cells within the epineurium of the sciatic nerve. Insets depict Lyve1⁺ cells in close proximity to CD31⁺Collagen IV⁺ blood vessels (filled arrowheads). Not all Lyve1⁺ cells are found to be associated with the blood vessels (open arrowheads). d, F4/80⁺Lyve^- endoneurial snMacs (open arrowheads) and F4/80⁺Lyve1⁺ epineurial snMacs (filled arrowheads) are depicted. Note that single F4/80⁺ epineurial snMacs were found to be Lyve1^- (see R2). (c,d), The perineurium and the basal membranes of blood vessels were stained with Collagen IV. Nuclei were counterstained with DAPI. Images are representative of three individual mice.