Extended Data Fig. 1: Gating strategy for sorting snMacs, onMacs and brain microglia for bulk RNA sequencing.
Top, representative gating strategy used to sort CD64+ snMacs (top) and purity check (down). The sciatic nerve from at least 5 mice were pooled and stained for the displayed surface markers. CD64+ cells among CD45+ cells were removed of doublets, dead cells, eosinophils, neutrophils and monocytes to obtain CD11bInt-HiCD64Hi snMacs. Bottom, representative gating strategies used to sort CD64+F4/80+ snMacs macrophages (top), CD45LoCD11bHi onMacs (middle) and CD45LoCD11bHi brain microglia (bottom) are displayed. The sciatic nerves and optic nerves were harvested from the same mouse and nerves of five individual animals were pooled for one biological replicate. Four individual biological replicates from independent experiments were submitted to RNA sequencing. For brain microglia, cells of five individual mice were sorted and submitted to RNA sequencing. Dump channel included antibodies against Ly6C, Ly6G, CD3e, CD19 and B220.
