Extended Data Fig. 4: Manipulation of CeAGA neurons did not induce anxiety-like or fear-like behavior or change the gross brain state.
From: General anesthetics activate a potent central pain-suppression circuit in the amygdala
a, Schematics of the Elevated Plus Maze (left) and Open Field (right) apparatus. b, Quantification of total time spent in the inner (GFP: 15.67 ± 5.97 s (baseline), 14.18 ± 7.24 s (stim), 18.07 ± 6.38 s (post); ChR2: 22.59 ± 4.92 s (baseline), 35.90 ± 10.22 s (stim), 29.73 ± 6.49 s (post); eArch: 5.69 ± 2.32 s (baseline), 11.01 ± 6.61 s (stim), 8.54 ± 5.20 s (post)) and outer perimeter (GFP: 284.32 ± 6.01 s (baseline), 285.70 ± 7.25 s (stim), 281.80 ± 6.39 s (post); ChR2: 277.31 ± 4.92 s (baseline), 264.00 ± 10.22 s (stim), 270.17 ± 6.47 s (post); eArch: 294.31 ± 2.32 s (baseline), 288.99 ± 6.61 s (stim), 291.46 ± 5.20 s (post)) of the Open Field Test (control, n = 8 animals, ChR2, n = 8 animals, eArch, n = 6 animals; two-way repeated measures ANOVA; P-value was above 0.05, no significance; F4,42 = 0.8743 (inner), F4,42 = 0.8633 (outer)). Data are mean ± s.e.m. c, Quantification of total distance travelled (GFP: 4.27 ± 0.73 m (baseline), 4.36 ± 0.72 m (stim), 3.36 ± 0.51 m (post); ChR2: 6.92 ± 0.87 m (baseline), 5.91 ± 0.88 m (stim), 5.85 ± 0.95 m (post); eArch: 4.59 ± 0.56 m (baseline), 5.45 ± 0.99 m (stim), 3.91 ± 0.59 m (post)), and d, total time spent in the open (GFP: 38.53 ± 12.26 s (baseline), 19.19 ± 6.65 s (stim), 11.03 ± 3.44 s (post); ChR2: 28.61 ± 9.69 s (baseline), 21.21 ± 6.31 s (stim), 27.91 ± 5.76 s (post); eArch (24.33 ± 4.70 s (baseline), 24.85 ± 4.98 s (stim), 14.35 ± 6.56 s (post)) and closed arms (GFP: 239.16 ± 17.28 s (baseline), 270.86 ± 8.54 s (stim), 280.76 ± 4.61 s (post); ChR2: 248.58 ± 11.66 s (baseline), 258.80 ± 9.36 s (stim), 261.08 ± 7.60 s (post); eArch (261.22 ± 4.07 s (baseline), 264.45 ± 5.50 s (stim), 272.48 ± 10.19 s (post)) of the Elevated Plus Maze (control, n = 8 animals, ChR2, n = 8 animals, eArch, n = 8 animals; two-way repeated measures ANOVA; P-value was above 0.05, no significance; F4,38 = 1.083 (distance), F4,38 = 1.402 (open arms), F4,38 = 1.355 (closed arms)). Data are mean ± s.e.m. e, Power spectrum of EEG signals in the frontal and parietal cortex. Left, laser on, right, laser off. f, Overlap of power spectrum of EEG signals in the frontal and parietal cortex from e. The mean spectrum in each condition was calculated from the average across 9 sessions (n = 3 mice, 2 min laser on / 2 min laser off, 3 repetitions in each mouse). The error bar represents the standard error. The power spectrum was normalized.
