Extended Data Fig. 8: CeAGA neurons are distinct from pain-activated neurons in CeA and high magnification image of CeAGA neurons projection into the ipsilateral BLA.
From: General anesthetics activate a potent central pain-suppression circuit in the amygdala
a, CeAGA neurons have minimal co-localization with formalin-induced Fos+ cells. Formalin-activated cells primarily locate in the capsular division of CeA outside the lateral division where CeAGA locate. Insert i)-v), Example of five consecutive slices of the lateral division of CeA showing minimal co-localization with formalin-induced Fos+ cells with CeAGA neurons, and the quantification of fraction of co-colocalization between CANE-captured CeAGA cells and formalin-induced Fos+ cells (n = 5 biologically independent samples for each condition) (0.78 ± 0.06 (Iso/Cane-Iso); 0.22 ± 0.04 (Form/Cane-Iso)). b, Coronal schematic next to example coronal slice of low magnification (high exposure) of CANE-GFP labeled CeAGA neurons and their axons with a box around the ipsilateral BLA. Insert i)-ii), High mag images show projections in ipsilateral BLA with top panel showing that isoflurane did not induce Fos+ cells, and bottom panel showing that formalin-pain induced robust Fos+ expression in BLA. (n = 3 biologically independent samples).
