Extended Data Fig. 5: Supporting information related to Figs. 4–5.

A) Changes in total water consumption (mean ± SD) expressed as the ratio of the total water volume (water+sucrose) in the SPT after LAN exposure to that before LAN exposure, corresponding to experiments in 4e (n = 7 mice for mCherry and n = 8 mice for Tet-tox), 4f (n = 6 mice for mCherry/CNO, n = 7 mice for hM4D/PBS, and n = 9 mice for hM4D/CNO), and 4n  (n = 9 mice for eGFP and n = 7 mice for Tet-tox). None was significantly altered by LAN exposure with p values of 0.0583, 0.6833, 0.1663, 0.9858, 0.3895, 0.2829, and 0.9468, respectively, as determined by two-tailed paired t-test comparing total consumptions pre vs. post within each group. B) (Related to experiments in Fig. 5a–d) Retrograde trans-synaptic tracing with RV revealed presynaptic inputs from IGL to NAc-projecting pHb neurons. The inter-geniculate leaflet (IGL, outlined) was densely innervated by ipRGCs. AAV2/2-EF1α-DIO-ChR2-eYFP was intravitreally injected to selectively express eYFP in ipRGCs, and intense labeling within the IGL was observed. dLGN: dorsal lateral geniculate nucleus. vLGN: ventral lateral geniculate nucleus. RV-labeled cells (arrowheads) were observed in the IGL. On average 26 ± 22 IGL neurons were observed on each side of each animal from a total of 6 mice. Bar: 100 μm.