Extended Data Fig. 2: Supporting information related to Fig. 2.

A) Schematic diagram of anterograde tracing with intravitreally-injected tracers. B) Retinal projections to the pHb area revealed by intravitreally-injected CTB-555 (b’) and HSV (b”), and ipRGC projections to the pHb revealed by AAV2-DIO-Channelrhodopsin (ChR2)-eYFP administered in Opn4-Cre mice (b”’). All tracing experiments were repeated at least three time. Scale: 100 μm. C) Schematic diagram of retrograde tracing from the pHb to retina. D) Retrogradely labeled (CTB, red) M1 ipRGCs (melanopsin), with dendrites stratified solely in the OFF sublamina of the retinal inner plexiform layer. Scale: 25 μm. E) Quantification of retrograde tracing results (contra: eye contralateral to the injection side; ipsi: ipsilateral eye; CTB: CTB only; Mela: CTB/melanopsin co-stained; n = 5 mice; contra: 18 cells; ipsi: 7 cells; Contra/mela: 75 cells; ipsi/mela: 6 cells). F) Subtype analysis of pHb-projecting ipRGCs judged from the stratification pattern (data were pooled from 4 retinae due to the difficulty of finding clearly stained dendrites and the cell body to which they belong on the same section. M1: 22 cells; M2: 2 cells; M3: 4 cells; Dislocated (DL): 2 cells). G) Fluorescence from CTB-555 at the injection site (pHb). Scale: 100 μm. H) Example of a M2 ipRGC that was stratified (arrowhead) in the ON sublamina of the retinal inner plexiform layer (IPL). Scale: 25 μm. I) Example of a M3 ipRGC that was stratified in both ON and OFF layers (arrowheads). Scale: 25 μm. J) Example of a dislocated ipRGC that appeared on the amacrine cell layer. Experiments in G-I were independently repeated four times in two mice. Scale: 25 μm.