Extended Data Fig. 3: Additional supporting information related to Fig. 2.

A) Histology for the optogenetic experiments in Fig. 2g–2i. Scale: 200 μm. B) Total water consumption (in grams/g, expressed as mean ± SD) corresponding to experiments in Figs. 2d and 2g, with p values of 0.4896, 0.2337 0.7212, and 0.9695, for the sham (n = 10 mice), lesion (n = 9 mice), viral (n = 19 mice), and ctrl (n = 13 mice) groups, respectively, as determined by two-sided paired t-test. C) Upregulation of cFos in the pHb elicited by the optostimulation of ipRGC terminals. Expression of cFos was induced by optostimulation of ipRGCs axonal terminals within the pHb. Selective expression of ChR2 in ipRGCs was achieved via intravitreal injection of AAV2/2-EF1α-DIO-ChR2-eYFP in Opn4-Cre mice (n = 5 sections). In sham-treated mice (littermates that received virus injection but no local opto-stimulation), minimum expression of cFos was observed (n = 6 sections). Parallel treated control mice in which virus injection was omitted did not respond to local optostimulation with elevated cFos expression (n = 7 sections). Bars: 50 μm. D) Schematic diagram of simultaneous retrograde tracing from NAc and mPFC using CTB-555 and CTB-488, respectively. E) Fluorescence at the injection sites: mPFC (green) and NAc (red). Experiments were independently repeated six times. Scale: 150 μm. F) Retrogradely-labelled NAc-projecting dpHb neurons (red dots) and mPFC-projecting vpHb neurons (blue dots) with rare overlaps (yellow dots). n = 6 mice. Data were expressed as mean ± SD. Scale: 100 μm.