Extended Data Fig. 4: Expression of Tak1 in the BA and perivascular macrophages in Cx3cr1CreER, Tak1Loxp/Loxp mice.
From: Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1
a, Male C57 BL/6 mice (12 weeks old) were perfused and then the brains were sectioned. Double immunofluorescence staining for Tak1 (red) and CD31, α-SMA or Vimentin (green) of basilar artery was performed. Cell nuclei were counterstained with DAPI (blue). Scale bars, 10 µm. n = 3 mice per group. b, Adult male Cx3cr1CreER, Tak1Loxp/Loxp and Cx3Cr1CreER, Tak1Loxp/Loxp (KO) mice were anesthetized and perfused with 4% PFA. Double immunofluorescence staining for Tak1 (red) and CD31 (green) of basilar artery was performed. Cell nuclei were counterstained with DAPI (blue). Scale bars, 10 µm. n = 3 mice per group. c, Double immunofluorescence staining for Tak1 (red) and CD11b (green, a macrophage marker). Arrows indicate double positive cells. Cell nuclei were counterstained with DAPI (blue). Scale bars, 10 µm. n = 3 mice per group.
