Extended Data Fig. 5: Effect of microglial Tak1 on energy balance, and the internal perimeter or elastin content in basilar artery.
From: Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1
a, Male Cx3cr1CreER, Tak1Loxp/Loxp and Cx3cr1CreER, Tak1Loxp/Loxp (KO) mice were fed a chow or high-fat diet (HFD) starting at 6 weeks of age. Body weight was then monitored. F(5, 196) = 31.6 for column factor. F(6, 196) = 27.7 for row factor. *P < 0.05 (HFD versus Chow). **P < 0.01 (HFD versus Chow). n = 5 (Chow), 6 (Cx3cr1CreER, HFD), 7 (Tak1Loxp/Loxp, HFD) or 6 (KO, HFD) mice per group. b,c, Fat mass (b) and lean mass (c) immediately before HFD treatment. n = 5 mice per group. d,e, Fat mass (d) and lean mass (e) after 24 weeks of chow or HFD treatment. In d, F(5, 38) = 6.52. *P < 0.05. n = 6 (Chow), 8 (Cx3cr1CreER, HFD), 9 (Tak1Loxp/Loxp, HFD) or 9 (KO, HFD) mice per group. (f) Cumulative food intake. n = 6 (Cx3cr1CreER, Chow), 7 (Tak1Loxp/Loxp, Chow), 6 (KO, Chow), 8 (Cx3cr1CreER, HFD), 10 (Tak1Loxp/Loxp, HFD) or 10 (KO, HFD) mice. g, Internal perimeter of basilar artery. n = 4 (Cx3cr1CreER, Chow), 3 (Tak1Loxp/Loxp, Chow), 4 (KO, Chow), 3 (Cx3cr1CreER, HFD), 3 (Tak1Loxp/Loxp, HFD) or 4 (KO, HFD) mice. h, EVG staining of basilar artery. Blue-black color represents elastin fibers. Scale bars, 20 μm. n = 5 (Cx3cr1CreER, Chow), 6 (Tak1Loxp/Loxp, Chow), 4 (KO, Chow), 6 (Cx3cr1CreER, HFD), 5 (Tak1Loxp/Loxp, HFD) or 5 (KO, HFD) mice. i, Relative elastin level in basilar artery. au, arbitrary unit. n = 5 (Cx3cr1CreER, Chow), 6 (Tak1Loxp/Loxp, Chow), 4 (KO, Chow), 6 (Cx3cr1CreER, HFD), 5 (Tak1Loxp/Loxp, HFD) or 5 (KO, HFD) mice. Data are presented as mean ± SEM. One-way (d) or two-way (a) ANOVA with Bonferroni’s post hoc test.
