Extended Data Fig. 5: Thalamic and layer 1 cortical areas targeted by the medial and lateral cerebellar nuclei.

a, Thalamic nuclei with monosynaptic inputs from the right medial nucleus (MN) and lateral nucleus (LN) identified via AAV1-mediated anterograde transsynaptic tracing. The number of the trans-synaptically labeled thalamic neurons in each nucleus indicated as + + (high), + (low), and - (none). b, Disynaptic inputs from the right MN and LN to distinct cortical regions of the left cerebral cortex, identified via transsynaptic tracing. Labeled input density to layer 1 of each cortical area is indicated as + + (high), + (low), and - (none). c, Representative tracing results of disynaptic inputs from the right MN and LN to distinct areas in the left cerebral cortex. Axons and terminals of thalamic neurons trans-synaptically labeled from injections to MN and LN are indicated in black. Results from injections to MN and LN are in the left and right, respectively, of each panel. Top and bottom parts of each panel correspond to layer 1 and 6. Inputs to layer 1 originate predominantly from the VM thalamus. Arrowheads indicate layer 1. Scale bar applies to all panels. d, representative injection site in the LN and e, representative injection site in the MN. Scale bar applies to all panels. Abbreviations; Aud, auditory cortex; Cg, cingulate cortex; CL, centrolateral thalamic nucleus; FrA, frontal association cortex; IL, infralimbic cortex; LD, laterodorsal thalamic nucleus; LO, lateral orbital cortex; LP, lateroposterior thalamic nucleus; M1 and M2, primary and secondary motor cortex; MD, mediodorsal thalamic nucleus; MO, medial orbital cortex; PF, parafascicular thalamic nucleus; Po, posterior thalamic group; PrL, prelimbic cortex; Ptl, parietal association cortex; Rhi, ecto-/peri-/ento-entorhinal cortex; Rsp, retrosplenial cortex; S1 and S2, primary and secondary sensory cortex; Tem, temporal association cortex; VL, ventrolateral thalamic nucleus; VM, ventromedial thalamic nucleus; VO, ventral orbital cortex.