Extended Data Fig. 6: ChR2 activation of VM thalamus-PRL mPFC circuit.
From: Regulation of autism-relevant behaviors by cerebellar–prefrontal cortical circuits
a, Sample injection site locations for ChR2/Arch injection into the left VM thalamus. b, To test for possible antidromic activation, in vivo anesthetized single unit recordings in the VM thalamus-targeted parietal association cortex (PAC) were performed upon mPFC laser stimulation of ChR2 or GFP VM-thalamus terminals at 20 Hz or 4 Hz. Image of TdTomato positive terminals in PAC from LN AAV-1 tracing injection (top left). c, Three chambered social approach assay; time spent sniffing novel animal (NA), or novel object (NO) with 20 Hz (left) or 4 Hz (right) stimulation. d, Three chambered social novelty testing; time spent sniffing NA, or familiar animal (FA) with 20 Hz (left) or 4 Hz (right) stimulation. e, Time in the open arm and distance traveled in the elevated plus maze assay. f, Distance traveled in the open field and g, time in the center of the open field. All behavioral tests were analyzed with two or three-way ANOVA and Sidak post test and recordings were analyzed with two way ANOVA (unmarked = not significant). For frequency of spiking shown in figures as normalized values (mean SEM), raw values can be found in Supplementary Fig. 12. Box line denoted median/whiskers denoted 5-95%. n ≥ 10 for all behavioral experiments. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. P values and complete animal numbers can be found in Supplementary Table 2.
