Extended Data Fig. 9: Gi DREADDs inhibition of PCs in the posterior vermis and Gq DREADDs activation of posterior vermis in PC-Tsc1 mutant mice.
From: Regulation of autism-relevant behaviors by cerebellar–prefrontal cortical circuits
a, Injection sites from MN and LN tracing to VM thalamus (top) and sample locations of Gi (inhibitory) or Gq (excitatory) DREADD injections into posterior vermis of PC-Tsc1 mice (bottom). b, Three chambered social approach assay; time spent sniffing novel animal (NA), or novel object (NO). c, Three chambered social novelty testing; time spent sniffing NA, or familiar animal (FA). d, Time in the open arm and e, distance traveled in the elevated plus maze assay. f, Time in the center of the open field and g, distance traveled in the open field. h, Latency to fall in accelerating rotarod test. i, Three chambered social approach assay; time spent sniffing novel animal (NA), or novel object (NO). j, Three chambered social novelty testing; time sniffing novel animal (NA), or familiar animal (FA). k, Time in the open arm and distance traveled in the elevated plus maze assay. l, Time in the center of the open field and distance traveled in the open field. m, Latency to fall in accelerating rotarod test. All behavioral tests were analyzed with two or three-way ANOVA and Sidak post test. Box line denoted median/whiskers denoted 5-95%. n ≥ 10 for all behavioral tests. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. P values and complete animal numbers can be found in Supplementary Table 2.
