Extended Data Fig. 4: Inhibition of LN in PC-Tsc1 mutant mice.
From: Regulation of autism-relevant behaviors by cerebellar–prefrontal cortical circuits
a, Sample injection site locations and injection site pictures of Gi DREADDs injected into LN of PC-Tsc1 mutant mice. b, Single unit activity in the right lateral nucleus (LN) with chemogenetic inhibition (Gi) or control GFP injection. c, Three chambered social approach assay; time spent sniffing novel animal (NA), or novel object (NO). d, Three chambered social novelty testing; time spent sniffing NA, or familiar animal (FA). e, Time in the open arm and distance traveled in the elevated plus maze assay. f, Time in the center of the open field and g, distance traveled in the open field. h, Latency to fall in accelerating rotarod test. All behavioral tests were analyzed with two or three-way ANOVA and Sidak post hoc testing. Recordings were analyzed with Mann-Whitney test, shown as mean SEM. For frequency of spiking shown in figures as normalized values, raw values can be found in Supplementary Fig. 12. Box line denoted median/whiskers denoted 5-95%. n ≥ 10 for all behavioral experiments. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. P values and complete animal numbers can be found in Supplementary Table 2.
