Extended Data Fig. 3: In situ hybridization of slc1a3b, kcnj10a/Kir4.1, and gfap in 3 dpf larvae.
From: Live-imaging of astrocyte morphogenesis and function in zebrafish neural circuits
a, Representative images show the comparison of slc1a3b, kcnj10a/Kir4.1, and gfap in the spinal cord. Dash lines mark the outline of spinal cord. Images are representative of N=3-4 fish larvae. Scale bars represent 500 μm for left panel, 200 μm for middle panel, and 20 μm for right panel, respectively. b, RNAScope in situ hybridization of kcnj10a in Tg[slc1a3b:myrGFP-P2A-H2AmCherry] fish spinal cord at 3 dpf. Single z-plane, dorsal view. Scale bar, 20 μm. Representative images from N=3 fish larvae. c, Double staining in situ hybridization of slc1a3b (purple) and gfap (red) at 3 dpf. Scale bar, 500 μm. Representative images from N=6 fish larvae.
