Extended Data Fig. 1: Optogenetic tagging of glutamatergic neurons.

a, The PO^Glu neurons expressing tdTomato in CaMKII-tdT mice. Scale bar, 200 µm. The white box depicting the area shown in the box of the PO. Scale bar, 20 µm. b, The PF^Glu neurons expressing tdTomato in CaMKII-tdT mice. Scale bar, 200 µm. The white box depicting the area shown in the box of the PF. Scale bar, 20 µm. c, An example spike sorting result from a single tetrode in the PO. d, An example spike sorting result from a single tetrode in the PF. e, Schematic illustration of electrophysiological recording in the PO of freely moving CaMKII-Cre mice with PO infusion of AAV-DIO-ChR2-mCherry. Enlargement showing optrodes. f, Typical image of AAV-DIO-ChR2-mCherry viral expression within the PO of CaMKII-Cre mice. Scale bar, 200 µm. g, Example recording of spontaneous and light-evoked spikes from a PO^Glu neuron. h, Overlay of light-evoked (blue) and averaged spontaneous (orange) spike waveforms from the example unit.