Extended Data Fig. 6: AAV9-SMN drives cytoplasmic aggregation of overexpressed SMN in motor circuit neurons of mouse models.
From: Gain of toxic function by long-term AAV9-mediated SMN overexpression in the sensorimotor circuit
a, ChAT and SMN immunostaining of L5 LMC MNs from uninjected and either AAV9-GFP (5) or AAV9-SMN (5) injected WT mice at P11 and P150. Scale bar=10 µm. b, Percentage of L5 LMC MNs with SMN aggregates in the same groups as in (a) at P300. Data represent mean and SEM (n = 3 animals). c, PV and SMN immunostaining of L5 DRG PNs from uninjected and either AAV9-GFP (5) or AAV9-SMN (5) injected WT mice at P11 and P150. Scale bar=10 µm. d, Percentage of L5 DRG PNs with SMN aggregates in the same groups as in (c) at P300. Data represent mean and SEM (n = 3 animals). e, ChAT and SMN immunostaining of L2 MNs from WT (SMNΔ7) mice and either AAV9-GFP (5) or AAV9-SMN (5) injected SMA mice at the indicated time points. Scale bar=10 µm. f, Percentage of L2 MNs with SMN aggregates in the same groups as in (e) at P190. Data represent mean and SEM (n = 3 animals). g, PV and SMN immunostaining of L2 DRG PNs from the same groups as in (e). Scale bar=10 µm. h, Percentage of L2 PNs with SMN aggregates in the same groups as in (g) at P190. Data represent mean and SEM (n = 3 animals).
