Extended Data Fig. 8: Sex and spatiotemporal differences in C4A co-expression.
a, Three different thresholds were tested, namely the number of total co-expressed genes at PCC > 0.4 and the number of C4A-positive and C4A-negative genes at FDR < 0.05. Males had more co-expressed genes than females regardless of the threshold metric used (n = 36, 38, 45, 47, 39, 45, 39, and 45 for frontal cortex, anterior cingulate cortex, hippocampus, caudate, putamen, cerebellum, hypothalamus, and nucleus accumbens, respectively; permutation test, P < 10−5). b, Similarly, frontal and anterior cingulate cortex were the two most connected regions for C4A regardless of the threshold metric used (n = 36, 38, 45, 47, 39, 45, 39, and 45 for frontal cortex, anterior cingulate cortex, hippocampus, caudate, putamen, cerebellum, hypothalamus, and nucleus accumbens, respectively; permutation test, P < 10−5). c, Leftward shift in co-expression peak was observed in SCZ cases compared to neurotypical controls across different threshold metrics (n = 30, 42, 57, 68, 47, and 32 for control samples in each age bin; n = 36, 46, 55, 45, and 47 for SCZ samples). All boxplots show median and interquartile range (IQR) with whiskers denoting 1.5 × IQR.
