Extended Data Fig. 2: cFos mapping in BLA inputs.
From: A thalamo-amygdalar circuit underlying the extinction of remote fear memories
Schematic representation of a, AAV2r injections into the BLA and b, behavioral groups used for cFos IHC in AAV2r-injected animals. Colocalization analysis of retrogradely traced cells from the BLA (AAV2r+) and cells activated upon remote fear memory recall and extinction (cFos+) in c, cortex: Two-way ANOVA, Control vs Extinction, F(1,39)=0.245, P = 0.62. Multiple comparison, Sidak. PL, N = 7 (No shock), 9 (Ext) mice/group; ACC, N = 8 (No shock), 9 (Ext) mice/group; IC, N = 6 (No shock), 6 (Ext) mice/group; d, midbrain: Two-way ANOVA, Control vs Extinction, F(1,46)=6.411, P = 0.0148. Multiple comparison, Sidak * P < 0.05. SUM, N = 6 (No shock), 19 (Ext) mice/group; VTA, N = 7 (No shock), 18 (Ext) mice/group; e, midline thalamus: Two-way ANOVA, Control vs Extinction, F(1,50)=3.989, P = 0.051. Multiple comparison, Sidak. Rh, N = 6 (No shock), 9 (Ext) mice/group; PVT, N = 8 (No shock), 7 (Ext) mice/group; IMD N = 7 (No shock), 6 (Ext) mice/group; CM, N = 8 (No shock), 7 (Ext) mice/group. f, hippocampus: unpaired t test P = 0.979. N = 7 (No shock), 8 (Ext) mice/group. ACC, anterior cingulate cortex; BLA, basolateral amygdala; CM, centromedial thalamus; IL, infralimbic cortex; IC, insular cortex (posterior, granular cortex); IMD, intermediodorsal nucleus of the thalamus; NRe, nucleus reuniens of the thalamus; PL, prelimbic cortex; PVT, periventricular thalamus; Rh, rhomboid nucleus; SUM, supramammilary nucleus; vCA1, ventral CA1; VTA, ventral tegmental area. Data are represented as mean ± SEM. Statistical analysis details for each figure panel are reported in Supplementary Table 1.
