Extended Data Fig. 2: Transcriptome analysis of dura-resident leukocytes during neuroinflammation in mice.
a, Merged Uniform Manifold Approximation and Projection (UMAP) plot of tissue-resident leukocytes (TRL) isolated from the dura merged of naive and EAE mice corresponding to the split UMAP plot depicted in Fig. 1h. b, Dotplot of marker genes defining the different cell clusters identified in the dura of naive and EAE mice as shown in A and in Fig. 1h. Dot size encodes percentage of cells expressing the gene, color encodes the average per cell gene expression level. c, Gene score feature plots depicting the expression of gene sets identified in ILC subtypes previously (Vivier et al. 2016; Robinette et al. 2015). d, Dot plot depicting the expression of plasma cell related genes in the Bc, cswBc, and BcProg cluster in healthy and EAE mice merged. Dot size encodes percentage of cells expressing the gene, color encodes the average per cell gene expression level. e, Stacked bar plots depicting cell cluster proportions in the scRNA-Seq dataset from naive vs. EAE mice as shown in Fig. 1h. f, Plots corresponding to Fig. 1i quantifying the change of cluster proportions in naive (left) vs. EAE (right) depicted on a linear scale to facilitate the log to linear transformation. g, Dot plot depicting the expression of plasma cell related genes in the Bc and cswBc cluster in naive vs. EAE mice. Dot size encodes percentage of cells expressing the gene, color encodes the average per cell gene expression level.
