Extended Data Fig. 4: Characterization of CSF and IgA+ B cells from naive vs. EAE mice.
a, Proportion (%) of Bc clones expressing any Ighg (for example Ighg2b, Ighg2c, Ighg3) gene and Igha, Ighd, Ighm genes as identified by BCR reconstruction from 3′ single cell RNA-sequencing libraries from naive vs. EAE mice (Methods). Bc clones were classified based on the number of cells expressing identical clonotype sequences into categories Single (0–1 cells), Small (2–5 cells), Medium (6–10 cells), Large (11–25 cells). Related to Supplementary Table 12. b, Total number of IgA+CD19+ Bc and percentage of IgA+CD19+ Bc of tissue-resident leukocytes identified by flow cytometry in the dura from naive vs. EAE mice. Data represented as mean ± SD, n = 6, two-sided Mann-Whitney U test was used to calculate statistical significance, *p < 0.05 **p < 0.01. c, Representative flow cytometry gating strategy to identify IgA expressing Bc in the dura of naive mice (top row) and in the dura of EAE mice (bottom row). d, Representative flow cytometry gating strategy to identify lymphocytes in the CSF of naive mice (top row) and EAE mice (bottom row). e, Quantification of Bc and Tc proportions in the CSF of naive mice corresponding to D. mean ± SD, n = 5, two-sided Mann-Whitney U test was used to calculate statistical significance (f) Quantification of Bc, Tc and CD4+ Tc in CSF of naive and EAE mice corresponding to D. mean ± SD, n = 5 naive, n = 6 EAE, two-sided Mann-Whitney U test was used to calculate statistical significance, P-value not depicted = not significant.
