Extended Data Fig. 7: Absolute time in NREM sleep following sleep deprivation is reduced in cortical SNAP25-ablated animals but relative NREM rebound does not differ between genotypes.
a) Time spent in vigilance states (wake, NREM, and REM) during the 18 h recovery time following sleep deprivation (genotype × vigilance state interaction: F(1,14) = 27.754, p < 0.001, mixed ANOVA). Note that cortical SNAP25-ablated animals (cKO) overall spent more time awake and less time in NREM and REM sleep compared to controls (CTR). b) Time course of NREM sleep on a sleep deprivation day compared between genotypes (genotype × time interaction: F(4,54) = 4.222, p = 0.004, mixed ANOVA). cKOs sleep less during the entire 12 h dark period following sleep deprivation. c) Rebound of NREM sleep time following sleep deprivation relative to individual baseline values. No differences were observed between genotypes. d) Change in duration of NREM episodes during the first hour after sleep deprivation (ZT6-7 of SD day) relative to the same time window on BL day. n = 6 CTR and n = 9 cKO for vigilance state analysis. Black asterisks indicate post-hoc contrasts with significant differences (*p < 0.05, **p < 0.01, ***p < 0.001). Data in panels a, c, d is presented as group mean (red line), 95% confidence interval (pink box), and one standard deviation (blue box) with individual data points overlaid. Data in panel b are presented as mean values ± SEM(shaded areas). See Supplementary Table 1 for detailed results. BL: baseline. cKO: conditional knockout animals. CTR: control animals. NREM: Non-rapid eye movement sleep. REM: Rapid eye movement sleep. SD: sleep deprivation. ZT: zeitgeber time.
