Extended Data Fig. 10: Passive infrared recordings (PIR) show robustness of sleep phenotype to altered light-conditions.
a) Wake time estimates averaged over the last 3 days of PIR recordings under 12:12 light-dark (LD) conditions and over the first 3 days of constant darkness (DD) (main effect of genotype: F(1,15) = 18.604, p = 0.001, mixed ANOVA). Note that genotype differences in the daily amount of wakefulness persist in the absence of light. b) Time course of wakefulness in LD and DD conditions (genotype × time interaction: F(3.801,57.016) = 3.319, p = 0.018, mixed ANOVA). n = 7 CTR and n = 10 for PIR recordings. cKO: conditional knockout animals. Black asterisks indicate post-hoc contrasts with significant differences (*p < 0.05, **p < 0.01, ***p < 0.001), grey asterisks indicate post-hoc comparisons with P < 0.05, which do not reach significance after Bonferroni correction for multiple comparisons. Data in panel a is presented as group mean (red line), 95% confidence interval (pink box), and one standard deviation (blue box) with individual data points overlaid. Data in panel b are presented as mean values ± SEM (shaded areas). See Supplementary Table 1 for detailed results. CTR: control animals. LD: light:dark. DD: constant darkness. PIR: passive infrared recordings.
