Extended Data Fig. 3: The suprachiasmatic nucleus of the hypothalamus is void of Cre + cells and spared of fibre tracts in the Rbp4-Cre driver line. | Nature Neuroscience

Extended Data Fig. 3: The suprachiasmatic nucleus of the hypothalamus is void of Cre + cells and spared of fibre tracts in the Rbp4-Cre driver line.

From: A role for the cortex in sleep–wake regulation

Extended Data Fig. 3: The suprachiasmatic nucleus of the hypothalamus is void of Cre + cells and spared of fibre tracts in the Rbp4-Cre driver line.

a) Epifluorescence image of an Rbp4-Cre;Ai14;Snap25fl/+ brain section at the level of the suprachiasmatic nucleus (SCN). The section was counterstained with DAPI (blue). Box indicates approximate region from which image in (b) was taken. b) High-magnification epifluorescence image of the SCN region. Rbp4-Cre;Ai14 axons are shown in red, cell nuclei stained with DAPI in blue. Note that there are no Cre+ cells located within the SCN (outlined with white dotted lines), and very few of the dense axon bundles pass through the SCN. DAPI: 4′,6-diamidino-2-phenylindole. SCN: suprachiasmatic nucleus. Scale bars: 1 mm (a), 100 µm (b).

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