Extended Data Fig. 6: Genotype differences in the amount of time spent in wake, NREM, and REM sleep during undisturbed baseline recordings are more pronounced in the dark period.
During the light period, the distribution of vigilance states is similar between genotypes with only a trend towards increased wakefulness and reduced NREM and REM sleep, while strong differences occur during the dark period (genotype × phase × vigilance state interaction: F(1,14) = 36.083, p < 0.001, three-way ANOVA). n = 6 CTR and n = 9 cKO for vigilance state analysis. Black asterisks indicate post-hoc contrasts with significant differences (*p < 0.05, **p < 0.01, ***p < 0.001), grey asterisks indicate post-hoc comparisons with P < 0.05, which do not reach significance after Bonferroni correction for multiple comparisons. Data is presented as group mean (red line), 95% confidence interval (pink box), and one standard deviation (blue box) with individual data points overlaid. See Supplementary Table 1 for detailed results. cKO: conditional knockout animals. CTR: control animals. NREM: Non-rapid eye movement sleep. REM: Rapid eye movement sleep.
