Extended Data Fig. 3: Heterogenous responses to noxious stimuli in LPB neurons.
From: Pain modulates dopamine neurons via a spinal–parabrachial–mesencephalic circuit
(a) Schematic of experimental design showing in vivo electrophysiological recordings of non-projection defined LPB neurons. Animals were exposed to tail pinch, heat, and electrical shock. (b) Pie graphs represent the proportion of LPB cells that are excited (green), inhibited (red) and do not respond (grey) to tail pinch, heat, or electrical tail shock (n = 63-97 cells from n = 5 mice). (c) Samples of spike raster plots and firing patterns for two different LPB cells that either excited (top) or inhibited (bottom) in response to noxious stimuli. Note that the top and bottom graphs are from the same LPB cell (that is, an LPB cell that was excited by all three noxious stimuli (top) and another LPB cell that was inhibited by all three noxious stimuli (bottom)). (d,e) Analysis for LPB cells that were recorded in response to all three noxious stimuli (n = 60 cells). Overlap between the proportion of LPB cells that were excited (top, green, n = 30 cells) or inhibited (bottom, red, n = 16 cells) in response to individual noxious stimuli (d) and LPB cells that show both excitatory and inhibitory responses for individual noxious stimuli (left, n = 9 cells) or did not respond at all (right, n = 5 cells) (e).
