Extended Data Fig. 9: SNR → LPB neurons have very few collaterals to NAcLat-projecting VTA DA neurons.
From: Pain modulates dopamine neurons via a spinal–parabrachial–mesencephalic circuit
(a) Schematic showing injection of fluorogold (FG) into the LPB and AAVs encoding the cellular receptor for subgroup A avian leukosis viruses (TVA) and rabies virus glycoprotein (RG) in the VTA of DAT-Cre mice. In the same animals, EnvA-pseudotyped, glycoprotein-deficient rabies virus expressing GFP (EnvA-RV-GFP) was targeted to the NAcLat. (b) Representative example of coronal section of the ventral midbrain showing retrogradely labeled cells in the lateral SNR (that is, LPB-projecting, FG-positive, red). GFP-positive cells (green) make monosynaptic connections onto VTA DA neurons projecting to NAcLat and are mainly located in the substantia nigra pars compacta (SNc), ventral SNR (vSNR) and lateral VTA, but do not overlap with the lateral SNR (lSNR; scale bar 100 µm). (c) Pie chart showing proportion of analyzed cells (n = 4035 cells from n = 3 mice) in the ventral midbrain that express GFP (green, 19.7%) or are labeled by FG (red, 79.4%) or contain both GFP and FG (yellow, 0.9%).
