Extended Data Fig. 10: Chemogenetic inhibition of RMTg-VTA GABAergic pathway does not alter sucrose preference in two-bottle choice test.
From: Pain induces adaptations in ventral tegmental area dopamine neurons to drive anhedonia-like behavior
a-b. Chemogenetic inhibition of RMTg GABA cells projecting to VTA does not alter sucrose preference or water consumption in two-bottle choice test. c. Sucrose consumption presented as percent change of baseline (two-way ANOVA for repeated measures, time: F1, 20 = 20.89, p = 0.0002; interaction (time x treatment): F2,20 = 6.236, p = 0.0079; Sidak’s post hoc within group: hM3Di + CFA + CNO(n = 8) versus hM3Di + CFA + VEH (n = 8), **** p < 0.0001) d. Representative coronal section of RMTg Gi DREADD expressing neurons. Blue – DAPI; Red – m-Cherry (Gi DREADD); Green – GAD 67 (GABA neurons). e. Representative coronal section of cannula placement in the VTA. Blue – DAPI; Red – m-Cherry (Gi DREADD); Green – TH (DA neurons). f. Spread of overlay of individual animal viral expression across RMTg in DREADD and control m-Cherry injected GAD-cre animals. i. Schematic representation of cannula placement in the VTA for local delivery of aCSF or CNO. The data are presented as the mean ± s.e.m.
