Extended Data Fig. 1: Fezf2^BLa neurons constitute a subset of pyramidal neurons in the BLa.

a, Confocal images of a coronal brain section containing the BLA from a Fezf2-Flp mouse, showing the distribution of Flp and Fezf2 detected by smFISH, and overlay as indicated. In the right-most panel are high magnification images of the boxed area in the overlay image. b, Quantification of the fraction of Flp⁺ neurons that co-express Fezf2, and vice versa (n = 3 mice). c, Confocal images of coronal brain sections from Fezf2-CreER;Ai14 mice, in which Fezf2⁺ neurons in the BLA are labeled with tdTomato (red). The BLa has the highest density of Fezf2⁺ neurons within the BLA complex. This experiment was repeated in 4 mice. d, Representative confocal images showing Fezf2^BLa neurons labeled with tdTomato (left), BLa pyramidal neurons labeled by antibodies recognizing CaMKII (middle), and overlay (right). Arrows indicate Fezf2^BLa cells expressing CaMKII. e, Representative images showing Fezf2^BLa cells labeled with tdTomato (left), BLa inhibitory neurons labeled by antibodies recognizing GABA (middle), and overlay (right). Arrows indicate Fezf2^BLa cells that do not express GABA. f, Quantification of the fraction of Fezf2^BLa neurons that are pyramidal neurons or GABAergic neurons (n = 4 mice). g, Quantification of the fraction of BLa pyramidal neurons that are Fezf2⁺ neurons or not (n = 4 mice). Data are presented as mean ± s.e.m.