Extended Data Fig. 2: Generation of PHOX2B::GFP reporter line and oMN-like cell specification in SOD1A4V and C9ORF72 ALS lines. | Nature Neuroscience

Extended Data Fig. 2: Generation of PHOX2B::GFP reporter line and oMN-like cell specification in SOD1A4V and C9ORF72 ALS lines.

From: Multi-omic analysis of selectively vulnerable motor neuron subtypes implicates altered lipid metabolism in ALS

Extended Data Fig. 2

(A-C′) Representative images and karyotype results of control hiPSC, SOD1A4V and C9ORF72 PHOX2B::GFP reporter lines. (D-E) Time course GFP expression of oMN-like differentiation in SOD1A4V and C9ORF72 by FACS analysis (SOD1A4V: n=3, C9ORF72: n=4 for each group; technical replicates; n means independent batches of differentiation). (F-K) Enrichment of transcripts in post-sorted ES-derived and both ALS-derived PHOX2B::GFP+ is comparable for oMN (ISL1, PHOX2A/B and NKX6.1), midbrain specification (EN1) and mDA specification (NURR1) by qRT-PCR analysis (D14) (at least n=3 for each group, n means independent batches of differentiation (independent sorted samples); technical replicates; n.s.: not significant, #P < 0.05 (0.0104), **P<0.01 (0.0097), two-tailed; unpaired student’s t-test). Scale bar: 100 μm, Error bars, mean ± SEM.

Source data

Back to article page