Extended Data Fig. 7: Validation of the predicted glomerular map. | Nature Neuroscience

Extended Data Fig. 7: Validation of the predicted glomerular map.

From: Spatial transcriptomic reconstruction of the mouse olfactory glomerular map suggests principles of odor processing

Extended Data Fig. 7

a, Light-sheet microscopic images of intrinsic fluorescent signals from the OBs of three independent mice harboring the Olfr73-IRES-GFP, Olfr160-IRES-tauCherry, or the Olfr1507-IRES-GFP alleles. Similar results were observed from each mouse line with at least 3 biological repeats per line. Scale bar: 500 μm. b, Reconstruction of the signals detected in a. The distance between the centroid positions of the same glomerulus detected in independent biological replicates is depicted. c, Example signal captured by MERFISH for each of the ORs depicted. Each spot represents an individual mRNA molecule that was detected by MERFISH in these experiments. From the three sections analyzed, OR signal was reliably detected in a total of 11 glomeruli, which is approximately the number expected given that ~300 OR probes were used in these experiments and the fact that sectioning the entire olfactory bulb at this thickness would result in ~300 sections. Scale bar: 500 μm.

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