Extended Data Fig. 4: UMAP projections reveal distinct transcriptomes expressed by different types of OSN.

a, Normalized expression of the Acsm4, Nrp2, Plxna1, and Nrp1 genes on UMAP projection plots of OSN scRNAseq data. b, UMAP plots of OSNs were regenerated with all OR gene expression information removed. OSNs expressing each of ten random ORs selected in Fig.2a are pseudocolored in the UMAP plot, revealing that cells displaying the same OR cluster together even in the absence of OR gene expression information. c, UMAP plots were regenerated in a pairwise manner using each of the groups of OSNs expressing the ten most frequently occurring ORs with OR gene expression information removed prior to clustering. In each pairwise analysis all of the OSNs expressing one OR are colored red, and all of the OSNs expressing the other OR are colored blue to make it easier to visualize their separation in UMAP space. d, ‘Shuffled’ control for the pairwise OSN UMAP segregation analysis. The analysis was performed as in c, except OR identity was randomly assigned prior to analysis. e, Analysis of all of the differentially expressed genes identified in a pairwise manner for each of the 654 types of OSN. For each differentially expressed gene, we determined for each of the 654 types of OSN in how many of those types of OSN is that gene detected in at least 10% of the sequenced cells.