Extended Data Fig. 4: Decoding and place field quality for different calcium indicators, fluorescence trace processing, and decoding methods.
From: Hippocampal place codes are gated by behavioral engagement
All panels are plotted as in Fig. 2h-i and Fig. 3e-f. a. jRGECO1a sessions. Two-sided Wilcoxon signed rank test: Engaged vs. disengaged for decoder trained on engaged trials, p = 4.4 × 10−4. Engaged vs. disengaged for decoder trained on disengaged trials, p = 0.03. Train/test on engaged vs. train/test on disengaged, p = 4.4 × 10−4. Two-sided Wilcoxon rank sum test: reliability p = 0, selectivity p = 9.64 × 10−200. n = 16 sessions, 4 mice. Shading represents mean ± SEM. b. jGCaMP8m sessions. Two-sided Wilcoxon signed rank test: Engaged vs. disengaged for decoder trained on engaged trials, p = 4.4 × 10−4. Engaged vs. disengaged for decoder trained on disengaged trials, p = 0.02. Train/test on engaged vs. train/test on disengaged, p = 2.3 × 10−3. Two-sided Wilcoxon rank sum test: reliability p = 0, selectivity p = 9.64 × 10−200. n = 16 sessions, 4 mice. Shading represents mean ± SEM. c. ΔF/F instead of deconvolved activity. Two-sided Wilcoxon signed rank test: Engaged vs. disengaged for decoder trained on engaged trials, p = 8.0 × 10−7. Engaged vs. disengaged for decoder trained on disengaged trials, p = 0.54. Train/test on engaged vs. train/test on disengaged, p = 1.1 × 10−6. Two-sided Wilcoxon rank sum test: reliability p = 0, selectivity p = 5.7 × 10−206. n = 32 sessions, 8 mice. Shading represents mean ± SEM. d. Template matching decoder. Two-sided Wilcoxon signed rank test: Engaged vs. disengaged for decoder trained on engaged trials, p = 8.0 × 10−7. Engaged vs. disengaged for decoder trained on disengaged trials, p = 0.54. Train/test on engaged vs. train/test on disengaged, p = 1.7 × 10−6. n = 32 sessions, 8 mice. Shading represents mean ± SEM.
