Fig. 2: Spatial localization of DA subtypes in NHP and human midbrain.

a, Nissl staining of a 10-μm M. fascicularis midbrain slice adjacent to Slide-seq-assayed tissue. Circles indicate approximate location of the placement of the three Slide-seq arrays shown in b–d. RN, red nucleus; CP, cerebral peduncles; SN_pcd, substantia nigra pars compacta dorsal; SN_pcv, substantia nigra pars compacta ventral. Cartesian arrows indicate orientation of bead arrays in b–d; scale bar, 1 mm. Nissl staining was repeated nine times across macaque brain. b–d, Bead arrays colored by RCTD cell type definitions (Methods) corresponding to major cell type (b), CALB1⁺ or SOX6⁺ subtypes (c) and the three most spatially variable DA subtypes (d). e, Ridge plot for aggregated densities of CALB1 and SOX6 subtypes (top) and all ten DA subtypes (bottom) across 27 bead arrays (Methods, also includes definition of midline). f, Tiled image of one 10-μm human midbrain slice. White dotted line indicates the approximate A9 region; scale bar, 1 mm. Experiment was repeated once. g, Scatter plots showing relative location of triple- (yellow) and single-positive cells (Methods) from in situ hybridization of markers: CALB1⁺/GEM⁺ (left), CALB1⁺/TRHR⁺ (middle) and SOX6⁺/AGTR1⁺ DA neurons (right); scale bars, 1 mm. Experiment was repeated five times for SOX6⁺/AGTR1⁺ localization and once for CALB1⁺/GEM⁺ and CALB1⁺/TRHR⁺.