Extended Data Fig. 10: Process of neurosphere formation and gene expression of cultured hippocampal neurospheres.
From: Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations
a-d) In vitro recording process of a cultured neurosphere clone from the adult macaque hippocampi. n = 4 clones. Scale bars, 40 μm. D, day. e) UMAP visualization of the cultured sphere cells (n = 6,988 cells), stained by the expression of selective marker genes. VIM, RGL and astrocyte marker; NNAT, novel neuroblast marker; SOX4, neuroblast marker; TUJ1, neuronal marker. Colorbar indicates log-normalized gene expression level. f-h) The cultured neurosphere cells from macaque hippocampus were immunoreactive for RGL and astrocyte marker VIMENTIN in f, neuroblast marker NNAT and SOX4 in g and h. n = 4 clones. Scale bars, 40 μm. i) Immunostaining analysis of EdU long-term labeling of the cultured neurospheres from the adult macaque hippocampus showed a small number of cells in the neurosphere were labeled by fluorescence for both TUJ1 and EdU in i (indicated by arrows). Scale bars, 40 μm. n = 4 clones for each staining. j) UMAP visualization of transcriptomic cell types projected from the Hochgerner_1 dataset. Red dots represent the distribution of the predicted cell type. IPC, intermediate progenitor cell; NB, neuroblast; GC, granule cell; Astro, astrocyte.
