Extended Data Fig. 7: Injury paradigms alter mrc1a+ microglia and expression of mammalian Mrc1 during injury.
(A) Quantification of spinal cord mCherry intensity in MTZ-treated animals across time from 1 to 4 dpi compared to DMSO treated zebrafish animals (t-test, two-tailed; multiple comparisons corrected: 1 dpi DMSO vs. MTZ p < 0.0001, 2 dpi DMSO vs. MTZ p < 0.0001, 3 dpi DMSO vs. MTZ p < 0.0001, 4 dpi DMSO vs. MTZ p < 0.0001; all two-tailed)(n = 70 animals). (B) Stacked violin plot of microglia from Hammond et al. (2019) comparing canonical microglia marker gene expression between Mrc1+ and Mrc1- microglia from P100 LPC-injected animals. Microglia were subsetted as Mrc1+ if log normalized expression of Mrc1 was > 0. (C) Differential expression testing results table comparing expression of canonical microglia markers in Mrc1+ versus Mrc1- microglia from Hammond et al. (2019). In (C) “Log Fold Change” refers to natural log fold-change, with positive values indicating higher expression in Mrc1+ microglia versus Mrc1- microglia. “Mrc1 + microglia with expression” and “Mrc1- microglia with expression” report the percentage of Mrc1+ and Mrc1- cells, respectively, with at least one read of the gene detected. Statistical significance was determined by Wilcoxon rank-sum test and an average log fold-change threshold of ±0.5. Imaging window equals 0.0027 mm3 (A).
