Extended Data Fig. 1: TgMOR; bgg10 mutants show impaired tolerance to fentanyl and morphine.
From: Ptchd1 mediates opioid tolerance via cholesterol-dependent effects on μ-opioid receptor trafficking
a, Time course showing animal speed after application of different concentrations of fentanyl (arrow) for tgMOR animals at 1st exposure (naïve animals). b, Time course of fentanyl doses for tgMOR after repeated (5x) exposure to negative control vehicle. c, Time course of fentanyl doses for tgMOR after repeated (5x) exposure to fentanyl (10 μM). d, Time course of fentanyl doses for tgMOR; bgg10 mutants at 1st exposure (naïve animals). e, Time course of fentanyl doses for tgMOR; bgg10 after repeated (5x) exposure to vehicle. f, Time course of fentanyl doses for tgMOR; bgg10 after repeated (5x) exposure to fentanyl (10 μM). g, Dose-response curves showing tgMOR animals develop tolerance after repeated (5x) exposure to morphine (300 μM). h, Quantitation of opioid efficacy (EC50 values from g) shows decreased morphine efficacy and development of tolerance by tgMOR animals after repeated (5x) exposure to morphine. For a-h, automated MWT was used to acquire all data. Plotted is mean data for n = 12 wells (4-5 animals/well) for all conditions and genotypes. Data for 12 wells were derived from 3 independent experiments. For g and h, data points and histograms are means. n = 3 independent experiments with each experiment derived from 4 wells (4-5 animals/well) per dose. For g, error bars are ± SEM. For h, error bars are ± SD and data were analyzed using one-way ANOVA and Bonferroni’s post hoc test. ***p < 0.001.
