Extended Data Fig. 2: RSBP-1/R7BP and FRPR-13 show differential effects on opioid tolerance.
From: Ptchd1 mediates opioid tolerance via cholesterol-dependent effects on μ-opioid receptor trafficking
a, Dose-response curves showing tgMOR; rsbp-1 mutants do not develop tolerance after repeated (5x) exposure to fentanyl (10 μM). b, Quantitation of opioid efficacy (EC50 values from a) shows similar fentanyl efficacy and absence of tolerance in tgMOR; rsbp-1 mutants after repeated (5x) exposure to fentanyl. c, Dose-response curves showing tgMOR; frpr-13 mutants develop tolerance after repeated (5x) exposure to fentanyl (10 μM). d, Quantitation of opioid efficacy (EC50 values from c) shows decreased fentanyl efficacy and development of tolerance by tgMOR; frpr-13 mutants after repeated (5x) exposure to fentanyl. For a-d, data points and histograms are means ± SD. n = 3 independent experiments with each experiment derived from 4 wells (4-5 animals/well) per dose for all genotypes. For b and d, data were analyzed using one-way ANOVA and Bonferroni’s post hoc test. ***p < 0.001.
