Extended Data Fig. 3: Altered opioid responsiveness of tgMOR; bgg10.
From: Ptchd1 mediates opioid tolerance via cholesterol-dependent effects on μ-opioid receptor trafficking
a, Representative traces of individual animal movement acquired using MWT after application of fentanyl (10 μM) for indicated time and genotypes. b, Time course of fentanyl response (10 μM, arrow) for indicated genotypes. Arrow denotes fentanyl application. Note only tgMOR and tgMOR; bgg10 animals respond to fentanyl. TgMOR; bgg10 mutants are hypersensitive reaching maximum paralysis after 45 minutes in fentanyl. For b, plotted is mean data for n = 12 wells (4–5 animals/well) for all genotypes. Data for 12 wells were derived from three independent experiments. Data were analyzed using two-way ANOVA and Bonferroni’s post hoc test. ***p < 0.001.
